Derivative Assay:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Modification:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Ubiquitin Proteomics:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Expressing:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Transfection:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Autoradiography:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Mutagenesis:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Construct:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Cell Culture:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Labeling:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Knockdown:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Incubation:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Control:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Staining:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Microscopy:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Conjugation Assay:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Mass Spectrometry:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Immunostaining:Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
Article Title: Endoplasmic Reticulum-associated Degradation of Niemann-Pick C1
Article Snippet: Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.Geldanamycin, anti-FLAG M2-agarose, and rabbit polyclonal anti-FLAG were from Sigma.. Rabbit polyclonal antibody against NPC1 was from Abcam and against Insig-1 (insulin-induced gene-1) from Biorbyt.. Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.Mouse monoclonal antibodies against ubiquitin (P4D1), heat shock protein 40 (Hsp40), Hsp70, Hsp90, heat shock cognate protein 70 (Hsc70), calnexin, HA tag, and GFP and rabbit polyclonal antibody against CHIP (carboxyl terminus of Hsp70-interacting protein) were from Santa Cruz Biotechnology.
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